| R64 | Matia Cristiani (BAB) def. Natalia Leroy (WES-W) 6-2, 6-4 |
| R64 | Lucy Mitchell (TUFTSWT) def. Ella Novion (COLBY) 6-1, 6-2 |
| R64 | Katie Driscoll (MIT) def. Mia Roets (WILW) 6-1, retired |
| R64 | Tina She (AMHW) def. Peyton Gaudreau (SPR-W) 6-2, 6-1 |
| R64 | Suzanne Pogorelec (BOW-W) def. Avni Bakre (BATW26) 6-2, 6-1 |
| R64 | Chahana Budhbhatti (BRANDEIS) def. Teah Login (EMERW) 6-0, 6-1 |
| R64 | Agnes Guggenheim (WES-W) def. I. G. Avendano (CSCW) 6-0, 6-1 |
| R64 | Elsie Van Wieren (MIDDW) def. P. Gonzalez Vallardi (NIC) 6-1, 6-0 |
| R64 | Anna Lardner (MIDDW) def. Cara Hung (BOW-W) 6-2, 3-6, 10-8 |
| R64 | Tina Liu (BAB) def. Avani Chitale (BRANDEIS) 6-1, 6-2 |
| R64 | Waka Aida (COLBY) def. Anwithan Duduka (AMHW) 6-4, 6-3 |
| R64 | Mariia Kornilova (WES-W) def. Heidi Cambron (SMIW) 6-1, 6-2 |
| R64 | Saina Deshpande (MIT) def. Alessandra Cristiani (BAB) 6-0, 6-1 |
| R64 | Diya Pachamuthu (MIDDW) def. Karolina Dobiecka (AMHW) 6-1, 6-2 |
| R64 | Mia Thomann (WILW) def. Elise Brennan (WTTC26) 7-6 (7-5), 6-1 |
| R64 | Melanie Woodbury (TUFTSWT) def. Asahi Goods (CCWT26) 6-2, 6-0 |
| R64 | Athena Li (TUFTSWT) def. Rebecca Suarez (BRANDEIS) 5-7, 6-3, 10-8 |
| R64 | Erica Jud (SPR-W) def. Amaya Briones (EMERW) 2-6, 6-4, 10-6 |
| R64 | Gusty Beveridge (MIDDW) def. Jolie Wang (BAB) 7-5, 6-3 |
| R64 | Lara Afolayanka (WES-W) def. Rayna Li (MIT) 6-2, 6-0 |
| R64 | Ayanna Varma (WILW) def. Chloe Zeng (MIT) 6-1, 6-2 |
| R64 | Zoe Sun (BOW-W) def. Ava Emrich (SMIW) 6-3, 6-1 |
| R64 | Sandra Sikharulidze (BAB) def. Payton Dugan (CSCW) 6-0, 6-0 |
| R64 | Lulu Wu (MIDDW) def. Maddie Swire (AMHW) 6-1, 2-6, 10-2 |
| R64 | Leila Epstein (WES-W) def. Caroline Stuhlfaut (BOW-W) 6-3, 6-2 |
| R64 | Kamora Helton (NIC) def. Charlotte McCann (MIDDW) 6-2, 1-6, 10-7 |
| R64 | Mandy Reyes (BRANDEIS) def. Isabella Saul (BATW26) 6-2, 6-0 |
| R64 | Sophie Diop (AMHW) def. M. Prendergast (CCWT26) 6-0, 6-1 |
| R64 | Ellie Gershaw (TUFTSWT) def. Allie Francis (WTTC26) 6-1, 7-5 |
| R64 | Ellie Stark (WILW) def. Michaela Landry (COLBY) 6-2, 6-2 |
| R64 | Sarah Youngberg (WES-W) def. Zainab Patel (UMB) 6-1, 6-0 |
| R64 | Fiona Lee (MIT) def. Bella Hartung (BOW-W) 4-1, retired |
| R32 | Matia Cristiani (BAB) def. Lucy Mitchell (TUFTSWT) 1-6, 6-0, 10-4 |
| R32 | Katie Driscoll (MIT) def. Tina She (AMHW) 7-6 (7-4), 1-6, 10-5 |
| R32 | Suzanne Pogorelec (BOW-W) def. Chahana Budhbhatti (BRANDEIS) 6-2, 6-0 |
| R32 | Elsie Van Wieren (MIDDW) def. Agnes Guggenheim (WES-W) 6-2, 6-2 |
| R32 | Tina Liu (BAB) def. Anna Lardner (MIDDW) 6-1, 7-6 (7-5) |
| R32 | Mariia Kornilova (WES-W) def. Waka Aida (COLBY) 6-3, 6-2 |
| R32 | Saina Deshpande (MIT) def. Diya Pachamuthu (MIDDW) 6-4, 6-3 |
| R32 | Melanie Woodbury (TUFTSWT) def. Mia Thomann (WILW) 5-7, 6-0, 10-5 |
| R32 | Athena Li (TUFTSWT) def. Erica Jud (SPR-W) 6-0, 6-0 |
| R32 | Gusty Beveridge (MIDDW) def. Lara Afolayanka (WES-W) 6-2, 7-5 |
| R32 | Zoe Sun (BOW-W) def. Ayanna Varma (WILW) 6-3, 6-3 |
| R32 | Sandra Sikharulidze (BAB) def. Lulu Wu (MIDDW) 6-2, 6-4 |
| R32 | Leila Epstein (WES-W) def. Kamora Helton (NIC) 6-2, 6-2 |
| R32 | Mandy Reyes (BRANDEIS) def. Sophie Diop (AMHW) 2-6, 6-2, 11-9 |
| R32 | Ellie Gershaw (TUFTSWT) def. Ellie Stark (WILW) 7-6 (7-0), 6-4 |
| R32 | Fiona Lee (MIT) def. Sarah Youngberg (WES-W) 6-2, 6-3 |
| R16 | Matia Cristiani (BAB) def. Katie Driscoll (MIT) 6-0, 6-0 |
| R16 | Elsie Van Wieren (MIDDW) def. Suzanne Pogorelec (BOW-W) 6-0, 7-5 |
| R16 | Tina Liu (BAB) def. Mariia Kornilova (WES-W) 3-6, 6-1, 6-1 |
| R16 | Saina Deshpande (MIT) def. Melanie Woodbury (TUFTSWT) 6-3, 6-2 |
| R16 | Gusty Beveridge (MIDDW) def. Athena Li (TUFTSWT) 3-6, 7-6 (7-3), 7-6 (7-4) |
| R16 | Zoe Sun (BOW-W) def. Sandra Sikharulidze (BAB) 6-3, 6-1 |
| R16 | Leila Epstein (WES-W) def. Mandy Reyes (BRANDEIS) 6-2, 3-6, 6-1 |
| R16 | Fiona Lee (MIT) def. Ellie Gershaw (TUFTSWT) 6-1, 6-3 |
| AQF | Matia Cristiani (BAB) def. Elsie Van Wieren (MIDDW) 6-2, 2-6, 6-4 |
| AQF | Saina Deshpande (MIT) def. Tina Liu (BAB) 7-5, 6-2 |
| AQF | Zoe Sun (BOW-W) def. Gusty Beveridge (MIDDW) 6-2, 6-7 (5-7), 6-1 |
| AQF | Fiona Lee (MIT) def. Leila Epstein (WES-W) 6-1, 4-6, 6-3 |
| ASF | Matia Cristiani (BAB) def. Saina Deshpande (MIT) 6-3, 6-4 |
| ASF | Fiona Lee (MIT) def. Zoe Sun (BOW-W) 6-0, 6-2 |
| AF | Matia Cristiani (BAB) def. Fiona Lee (MIT) 6-0, 6-2 |
| B64 | Dana Kamal (BAB) def. Jahnvai Vaghasia (WTTC26) 6-3, 6-2 |
| B64 | Natalie Hu (TUFTSWT) def. Emelyn Harris (BATW26) 6-1, 6-4 |
| B64 | Rachel Roth (AMHW) def. Anastasia Samets (MIT) 6-0, 5-7, 10-8 |
| B64 | Natazya Simunyola (SMIW) def. Kat Lowy (COLBY) 6-2, 7-5 |
| B64 | Sameera Talwar (AMHW) def. Milana Sofizade (BRANDEIS) 3-6, 6-2, 10-7 |
| B64 | Naomi Williams (BOW-W) def. Kendall Cedar (WTTC26) 7-5, 6-2 |
| B64 | Hannah Farber (BATW26) def. Sky Jara (CSCW) 7-6 (7-3), 7-6 (7-4) |
| B64 | Elizabeth Doak (MIDDW) def. Madison Damond (WTTC26) 6-0, 6-0 |
| B64 | Charly Saltz (BRANDEIS) def. Emilia Romano (WTTC26) 6-1, 6-1 |
| B64 | Soumya Wijesekera (COLBY) def. Lila Moldenhauer (BOW-W) 6-1, 7-5 |
| B64 | Riya Matharoo (BAB) def. Teagan Skulley (CCWT26) 7-5, 6-1 |
| B64 | Lia Swire (BATW26) def. Olivia Chin (SMIW) 7-5, 6-2 |
| B64 | Maya Muhunthan (AMHW) def. Grace Starcher (BAB) 6-4, 7-6 (7-2) |
| B64 | Emma Bazemore (WTTC26) def. Sana Bhat (CCWT26) 1-6, 6-3, 10-7 |
| B64 | Ryan Wuhrman (TUFTSWT) def. Manuela Moscoso (BAB) 7-5, 7-6 (7-5) |
| B64 | W. Assante-LaBash (BOW-W) def. Alysa Elliott (SPR-W) 6-0, 6-2 |
| B32 | Dana Kamal (BAB) def. Marie Frati (BOW-W) 6-4, 4-6, 10-4 |
| B32 | Jane Ladaga (WILW) def. Natalie Hu (TUFTSWT) 7-5, 6-7 (4-7), 10-8 |
| B32 | Lucinda Gatsiounis (WES-W) def. Rachel Roth (AMHW) 7-5, 7-5 |
| B32 | Nancy Zhang (BRANDEIS) def. Natazya Simunyola (SMIW) 6-2, 6-4 |
| B32 | Claudia Grove (TUFTSWT) def. Sameera Talwar (AMHW) 6-2, 6-2 |
| B32 | Naomi Williams (BOW-W) def. Tina Barrow (COLBY) 6-2, 7-5 |
| B32 | Alaina Kolli (MIT) def. Hannah Farber (BATW26) 6-0, 6-0 |
| B32 | Elizabeth Doak (MIDDW) def. Mia Sorrentino (BAB) 4-6, 7-6 (7-4), 10-1 |
| B32 | Cody Huang (AMHW) def. Charly Saltz (BRANDEIS) 2-6, 6-4, 11-9 |
| B32 | Soumya Wijesekera (COLBY) def. Amelie Gal (COLBY) 6-2, 6-1 |
| B32 | Riya Matharoo (BAB) def. Alyssa Khou (TUFTSWT) 6-1, 6-3 |
| B32 | Katelyn Wu (WILW) def. Lia Swire (BATW26) 6-0, 6-2 |
| B32 | Maya Muhunthan (AMHW) def. Anna Rende (COLBY) 6-2, 6-2 |
| B32 | Kendall Smith (WES-W) def. Emma Bazemore (WTTC26) 6-1, 6-0 |
| B32 | Ren Barton (MIDDW) def. Ryan Wuhrman (TUFTSWT) 7-6 (7-4), 6-1 |
| B32 | Carley Chen (MIT) def. W. Assante-LaBash (BOW-W) 1-6, 7-6 (7-2), 10-6 |
| B16 | Jane Ladaga (WILW) def. Dana Kamal (BAB) 6-2, 6-2 |
| B16 | Nancy Zhang (BRANDEIS) def. Lucinda Gatsiounis (WES-W) retired |
| B16 | Claudia Grove (TUFTSWT) def. Naomi Williams (BOW-W) 6-4, 3-6, 10-7 |
| B16 | Alaina Kolli (MIT) def. Elizabeth Doak (MIDDW) 6-4, 6-3 |
| B16 | Soumya Wijesekera (COLBY) def. Cody Huang (AMHW) 6-4, 6-3 |
| B16 | Katelyn Wu (WILW) def. Riya Matharoo (BAB) 6-1, 5-7, 10-8 |
| B16 | Kendall Smith (WES-W) def. Maya Muhunthan (AMHW) 6-2, 6-3 |
| B16 | Ren Barton (MIDDW) def. Carley Chen (MIT) 5-7, 6-0, 10-8 |
| BQF | Nancy Zhang (BRANDEIS) def. Jane Ladaga (WILW) 6-7 (4-7), 6-3, 11-9 |
| BQF | Alaina Kolli (MIT) def. Claudia Grove (TUFTSWT) 6-4, 6-4 |
| BQF | Soumya Wijesekera (COLBY) def. Katelyn Wu (WILW) 6-4, 6-1 |
| BQF | Ren Barton (MIDDW) def. Kendall Smith (WES-W) 6-4, 1-2, retired |
| BSF | Nancy Zhang (BRANDEIS) def. Alaina Kolli (MIT) walkover |
| BSF | Ren Barton (MIDDW) def. Soumya Wijesekera (COLBY) 6-1, 6-2 |
| BF | Ren Barton (MIDDW) def. Nancy Zhang (BRANDEIS) 6-0, 3-6, 11-9 |
